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Primer design for quantitative real-time PCR for the emerging Coronavirus SARS-CoV-2
Rebecca and John Moores UCSD Cancer Center DNA Sequencing Shared Resource
PhiSiGns | Manual
Primer Design for PCR
The web-based multiplex PCR primer design software Ultiplex and the associated experimental workflow: up to 100- plex multiplicity | BMC Genomics | Full Text
Using QuickTest Primer to check for hairpins in sequences, and not just primers.
IMPROvER: the Integral Membrane Protein Stability Selector | Scientific Reports
Calculating the melting temperature of PCR primers
Rebecca and John Moores UCSD Cancer Center DNA Sequencing Shared Resource
How is the minimum dG dimerization calculated on Primer Explorer for LAMP primer design? | ResearchGate
The Four Most Common Problems in Multiplex pcr Design | DNA Software
A Guide to LAMP primer designing
The web-based multiplex PCR primer design software Ultiplex and the associated experimental workflow: up to 100- plex multiplicity | BMC Genomics | Full Text
Primer Design
OligoAnalyzer Tool - Instructions for use | IDT
PhiSiGns | Manual
Article 3: qPCR Assay Design - European Pharmaceutical Review
Is it a problem to have a positive dG in primer self dimer? | ResearchGate
Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: Report of the BIOMED-2 Concerted Action BMH4-CT98-3936 | Leukemia
PrimerROC: accurate condition-independent dimer prediction using ROC analysis | Scientific Reports